Impact of HA-CMC barrier on ovarian s.c. tumor xenografts. (A) SKOV-3 cells were injected subcutaneously into the flanks of immunosuppressed mice at day 0 (D0). At day 1 (D1), a 1 cm2 piece of HA-CMC barrier was inserted into one flank of each mouse (HA-CMC barrier), the other flank being simply opened and closed again (control). Tumor volume was extrapolated from the measurement of tumor length and width every 2 or 3 days. (Mean tumor volume +/− SEM) 23 days after the injection, mice were sacrificed and tumors were sampled for histological analysis. (B) Mitotic index was determined by counting the number of mitoses per 10 high-power fields. (C) Ki67 positive cells (%) were determined by Ki67 marking.