Figure 5
Impact of HA-CMC barrier on ovarian i.p. tumors xenograft. (A) SKOV-3 cells were injected intraperitoneally into the abdomen of immunosuppressed mice. The day following the injection, half of the mice received a āwhiteā laparotomy (control) and the other half underwent a laparotomy with the insertion of a 2.25Ā cm2 piece of HA-CMC barrier (HA-CMC barrier). 21Ā days after the injection, mice were sacrificed and a macroscopic analysis was performed in order to evaluate the median peritoneal carcinosis index for each of diaphragm, liver, peritoneum and digestive tract. (Median +/ā Extreme values, *pā<ā0.05) Tumors were sampled for histological analysis. (B) Mitotic index was determined by counting the number of mitoses per 10 high-power fields. (C) Ki67 positive cells (%) were determined by Ki67 marking.