Figure 1

Effect of the induced expression of Spry1 on cell viability in SKOV-3 human ovarian cancer cells as compared to the negative controls. The two control groups include the SKOV-3 cells transfected with pcDNA3.1 plasmid (+vector, Genticin resistant) and the SKOV-3 cells with no plasmid transfection (−vector, Genticin sensitive). A. Western blot analysis of the protein expression in the Spry1-transfected cells as compared to the controls. Results show expression of Spry1 examined at 8 (8 h-trans), 24 (24 h-trans), 48 (48 h-trans) and 72 hours (72 h-trans) post-transfection. The Spry1-expressing cell line 1A9 was used as a positive control for the protein expression. GAPDH blot is shown as the loading control of the experiment. B. Crystal violet staining of the Spry1-transfected, +vector and -vector SKOV-3 cells after 2 weeks under Geneticin selection. As seen, no colony was formed by the Spry1-transfected and -vector cells. C. Trypan blue assay and; D. MTT assay, on Spry1-transfected cells on days 1 (a), 2 (b) and 3 (c) post transfection as compared to the negative controls. MTT assay cell viability results are shown as optical density (OD) units which are linearly correlated with the cell number. Both assays indicated a significant decrease in growth and proliferation of the Spry1-transfected cells on day 3 post-transfection. Images are representative of three independent experiments. Data are shown as mean ± SE. Significant values (<0.05) are marked by asterisks.