Figure 2

Spry1-induced inhibition of wound healing, migration and invasion in SKOV-3 cells. A. Scratch assay imaged at 0, 20 and 24 hours after scratch (left). Data analysis showed a significant decrease in the percent closure by the Spry1 transfection group as compared to the negative controls (+vector and –vector) 20 and 24 hours after scratch (right). B. Migration assay using 24-well Transwell system (left), showing a significantly lower number of the migrated cells in the transfection group 6 and 12 hours after plating (right). C. Invasion assay using matrigel-coated Transwell plates imaged 6 and 12 hours after plating (left), demonstrating a significantly lower number of the invaded cells in the transfection group assayed at the two endpoints (right). Images are representative of three independent experiments. Data are shown as mean ± SE. Significant values (<0.05) are marked by asterisks.