Knocking-down AQP5 decreases 3AO cells proliferation. 3AO cells infected with AQP5 shRNA lentivirus and various controls. The CCK8 assay demonstrated that cells treated with AQP5 shRNA have lower viability compared with the controls. The experiment was repeated for three times. Statistic significant differences were observed between cells infected with AQP5 shRNA virus and NC/mock cells.