Figure 2
Representative photomicrographs of normal and cancerous ovarian tissue sections showing AQP5-immunostained cells. Bouin's solution-fixed ovary tissue sections were immunostained using anti-human AQP5 antibody. AQP5 immunostaining (brown color) was visualized using a peroxidase-based detection system. A-C. Normal ovarian tissue sections exhibiting localization of AQP5 in granulosa and theca cell layers (arrows in A and B) and in ovarian surface epithelium (C); D-L. Cancerous ovarian tissue sections exhibiting localization of AQP5 in ovarian surface epithelium (arrow in D), borders around cysts (E, F), acini (arrow in G and H), theca cell layer (arrow in I), and in lobules of poorly differentiated neoplastic cells (arrows in J and K). Omitting anti-AQP5 antibody during the staining procedure abolished immunostaining completely (L). GCL- granulosa cell layer, TCL-theca cell layer, OSE-ovarian surface epithelium, CS-cystic space, Ac-acinus.