Figure 3

The flow cytometry test results of primary tumor cells after different fluorescent antibody labeling. The window of A contained live tumor cells to be sorted, and cells in B window were obtained from A window, which were FITC-negative and PE-negative cells. Cells in window of C were obtained from B window, where cells inside the window or on the left of the horizontal line were FITC-, PE- and CA125- negative cells, while cells outside the window or on the right of the horizontal line were FITC-negative, PE-negative and CA125-positive cells. Plots in D, E and F were testing results of sorted negative cells. Compared with A window, dead cells and cell debris in D window significantly reduced. Cells in E window were obtained from D window, which detected the purity of FITC- and PE-negative cells, while cells in F window were obtained from D window, which detected purity of FITC-, PE- and CA125-negative cells.