Figure 3
The electron micrograph of granulosa cells of secondary follicles. A) non-vitrified ovarian tissue, B, C) vitrified in double cryoprotectant, TEM: ×2156, D) theca cell of vitrified ovarian tissue in double cryoprotectant, TEM: ×3597. The intact contact c between granulosa cells GC is seen. The nucleus N and cytoplasmic organelles, mitochondria m, rough and smooth endoplasmic reticulum SER and RER are well defined. Theca cells TC in vitrified ovaries by double cryoprotectant contained collagen fibers CF is shown D.