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Table 1 Number and percentage of intact (Int) and degenerated (Deg) follicles of various stages isolated from vitrified and non-vitrified ovarian tissue after Trypan blue staining

From: Preservation of mouse ovarian tissue follicle morphology and ultra-structure after vitrifying in biotechnological protocols

Group

Total number of follicles

Number of primordial follicles (%)

Number of primary follicles (%)

Number of secondary follicles (%)

Ā Ā 

Total

Int

Deg

Stat

Total

Int

Deg

Stat

Total

Int

Deg

Stat

Control

259

73

72 (99)

1 (1)

-

91

88 (98)

2 (2)

-

95

93 (98)

2 (2)

-

CV1

232

75

69 (93)

6 (7)

-

84

73(86)

11 (14)

a, b

73

62 (83)

11 (17)

a, b

CV2

275

84

79 (94)

5 (6)

-

96

85 (88)

11 (12)

a, b

95

81 (85)

14 (15)

a, b

CV3

257

81

5 (92)

6 (8)

-

89

79 (89)

10 (11)

a, b

87

75 (85)

12 (15)

a, b

CV4

243

69

63 (91)

6 (9)

-

100

90 (90)

10 (10)

a

74

62 (82)

12 (18)

a, b

CV5

233

88

83 (95)

5 (5)

-

83

78 (94)

5 (6)

-

62

55 (88)

7 (12)

a, b

CV6

227

68

65 (95)

3 (5)

-

86

82 (95)

4 (5)

-

73

68 (93)

5 (7)

-

CV7

266

85

82 (97)

3 (3)

-

90

87 (97)

3 (3)

-

91

89 (98)

2 (2)

-

  1. aPā€‰<ā€‰0.05 versus non-vitrified control group.
  2. bPā€‰<ā€‰0.05 versus vitrified tissue in CV7 group.
  3. CV, Conventional vitrification.