Figure 2
From: Very small embryonic-like stem cells are the elusive mouse endometrial stem cells- a pilot study
Detection of VSELs in mouse uterus. Panel A Hematoxylin and Eosin stained uterine smears prepared after enzymatic digestion of the tissue. Note the presence of distinct spherical stem cells with dark stained nucleus with high nucleo-cytoplasmic ratio. In contrast, the somatic uterine cells have pale stained nucleus surrounded by abundant cytoplasm. Please note that 5-6 different fields have been combined to make this figure. Scale bar represent 20 μm. Lower Panel B shows RT-PCR results for pluripotent markers Oct 4A, Nanog, Sca-1, Oct 4 and Gapdh in bilaterally ovariectomized uterus (Gp A). Results show that pluripotent stem cells exist in atrophied endometrium.