Figure 4
Overexpression analysis of LAPTM4B in HEK cells. LAPTM4B was cloned into the his-tag pQE2 vector and used to transfect HEK cells. Proteins were extracted and subjected to immunoblotting using the anti-bovine (LA)2-LAPTM4B polyclonal antibody (panel A) or a commercially available anti-LAPTM4B antibody (panel B). Two proteins of 26.3 and 31.5Â kDa were observed with both antibodies. His-tag LAPTM4B proteins produced by HEK cells were purified by affinity chromatography and subjected to immunoblotting using a bovine ubiquitin antibody that only recognized the 31.5Â kDa LAPTM4B form (panel C).