Fig. 3

qRT-PCR validation of eight miRNAs in different sized follicle. qRT–PCR of selected known miRNAs in different size follicles (100 μm −130 μm, 200 μm -280 μm, 450 μm -550 μm, 500 μm -600 μm). miRNA was isolated and cDNA was synthesized from different size follicles followed by qRT-PCR. Capital letters shows expression profile of respective miRNA in deep sequencing data while small letters represents their expression profile in different size follicles using qRT-PCR. A) Expression profile of mmu-mir-199a in sequencing data. a) Expression profile of miR-199a through qRT-PCR. B) Expression profile of mmu-mir-470 in sequencing data. b) Expression profile of miR-470 through qRT-PCR. C) Expression profile of mmu-mir-871in sequencing data. c) Expression profile of miR-871 through qRT-PCR. D) Expression profile of mmu-mir-351 in sequencing data. d) Expression profile of miR-351 through qRT-PCR. E) Expression profile of mmu-mir-191 in sequencing data. e) Expression profile of miR-191 through qRT-PCR. F) Expression profile of mmu-mir-34c in sequencing data. f) Expression profile of miR-34c through qRT-PCR. G) Expression profile of mmu-let-7a in sequencing data. g) Expression profile of let-7a through qRT-PCR. H) Expression profile of mmu-mir-7a in sequencing data. h) Expression profile of miR-7a through qRT-PCR