Fig. 6

TQ/cisplatin combination increases pH2AX(ser139) expression in ID8-NGL-derived tumors. a Representative fields showing pH2AX(ser139) staining (green) and DAPI-stained nuclei (blue) in tumors harvested from ID8-NGL-injected mice treated with vehicle, TQ (20 mg/kg), cisplatin (2 mg/kg) or the TQ/cisplatin combination for 30 days. b The percentage of tumor cells positive for pH2AX(ser139) in drug-treated tumors. At least 200 cells were counted (×40 objective) for each drug treatment. c Western Blot analysis of pH2AX(ser139) expression in tumors. Total H2AX was used as a loading control. d Densitometry analysis of pH2AX(ser139) expression in tumors relative to corresponding H2AX levels. Values are mean + SD for 3–5 mice per group; *p < 0.01 relative to vehicle; all p values determined by Mann–Whitney test