Fig. 3

In established ovarian cancer cell lines, BECN1 copy-number correlates with mRNA and protein expression. a Log2-transformed mRNA expression data for 52 ovarian cancer cell lines [Cancer Cell Line Encyclopedia (CCLE) datasets] are displayed as a function of BECN1 copy number calls. Cell lines representative of low (CaOV3), intermediate (OVCAR8), and high (SKOV3, Hey A8) Beclin-1 expression were selected for further analysis. b Beclin-1 protein expression is depicted for the selected cell lines [normalized linear Reverse-Phase Protein Array (RPPA) values from The Cancer Proteome Atlas (TCPA) datasets]. c Independent protein lysates from adherent cultures (n = 3) were run on a single gel with representative lanes depicted for each cell line (intervening lanes cropped). d Beclin-1 expression was quantified relative to Actin. Bars: Mean ± SEM. One-way ANOVA with Newman-Keuls correction for multiple comparisons was performed. Letters denote statistically significant differences (n = 3 lanes per cell line, p < 0.05)