Fig. 1

Immunolocalization and immunoexpression of ERs in the ovaries of mice on CR (a, c, e) and in the ovaries of mice fed ad libitum (b, d, f). The high expression of ERs was observed in ovaries of mice on CR in granulosa cells (black arrow), theca cells (red arrow), ovarian surface epithelium cells (black arrowhead), interstitial cells (black asterisk), and endothelial cells (red asterisk) (a, c, e) compared with expression of ERs in the ovaries of control mice fed ad libitum in granulosa cells (black arrow), ovarian surface epithelium cells (black arrowhead), interstitial cells (black asterisk), and endothelial cells (red asterisk) (b, d, f). There was no immunoreaction in theca cells (b, f; red arrow) and in luteal cells (d; blue arrow) in the ovaries of mice fed ad libitum (b, d, f). Objective magnification: a, b, e, f, x20; c, d, x40