Fig. 6

MiR-31 down-regulates AFF1, reduces SEOC cell proliferation, colony formation, invasion and migration. a The three predicted locations of miR-31 target binding sites on AFF1 3’UTR. The vertical lines denote the “seed” regions by Watson-Crick base pairing. The colon (:) symbol denotes wobble base pair between guanine (G) and uracil (U) nucleotides. b The AFF1 expression was negatively regulated by miR-31 when measured with qPCR. c MiR-31 significantly reduced cells proliferation and inhibiting miR-31 markedly induce proliferation of SEOC cell lines. d Introduction of exogenous miR-31 into SEOC cells significantly inhibited clonogenic potential while (e) inhibition of miR-31 exhibits the opposite effect. Representative images of crystal violet stained colonies in each treatment were shown. f The cell invasion was assessed using pre-coated ECMatrix™ transwell membranes. g The cell migration assay using transwell membrane showed SEOC-miR-31 transfected cells were significantly less than that of SEOC-negative controls transfected cells. (H) Inhibition of miR-31 reversed the effects and enhanced SEOC cells migration. (*p < 0.05, **p < 0.01, ***p < 0.001, t-test)