Fig. 3
Comparison between cisplatin treatment in s.c. and i.p. xenografts. a A2780-Rab25 cells were injected at 2 × 106 subcutaneously (s.c.) or (b) 5 × 106 intraperitoneally (i.p.) in nude mice. Mice were left for approx. 1 weeks (s.c.) or 3–4 weeks (i.p.) to allow tumours to develop. Cisplatin (6 mg/kg, Cisplatin) was injected s.c. on day 1. Luciferase signal was monitored over time beginning day 0. SEM bars are shown. c Luminescent signal (arbitrary units) at day 14 compared to their relative luminescent starting signal at day zero. d In vitro increase in endogenous p53 expression following treatment of cells with 1uM cisplatin. e Overnight culture of cells on 24 well plates was incubated for 24 hours with cisplatin (4 wells per concentration). Luciferase activity was measured using the Xenogen IVIS and the cells counted. Luciferase activity is expressed as total flux × 103 per 1000 cells. f Mice (12) were injected i.p. with 5 × 106 A2780Rab25p53-Luc cells. Tumours developed over 27 days. The mice were imaged before and 24 hours after treatment with cisplatin 6 mg/Kg i.p. (6 mice in each group). Representative example of bioluminescent imaging signal. Graph depicts relative increase in p53 response luminescent signal