Fig. 4

Electron micrographs of oocytes cultured for, (a) 12 h in the absence of metformin from the PCOS-induced (1500×); (b) 12 h in the presence of metformin from the PCOS-induced, the arrows denote the cortical granules (8900×); (c) 24 h in the presence of metformin from the normal, the arrows denote the cortical granules (3200×); (d) 24 h in the presence of metformin from the PCOS-induced, degenerated cumulus cells were noticed. (1650×); (e) 36 h in the absence of metformin from the normal, degenerated cumulus cells were noticed. (3900×); (f) 36 h in the presence of metformin from the normal rats, PVS filled by microvilli. (1150×). ZP, zona pellucida; CC, cumulus cells; Oo, oocyte; Mt, mitochondria; PVS, perivitelline space; MV, microvilli