Fig. 4

Temporal expression of candidate circulatory miRNAs in blood plasma across estrous days. Relative expression of candidate circulatory miRNAs in blood plasma of hypersitimulated heifers across different days during estrous (a-f) Expression of miR-221, miR-103, let-7g, miR-134, miR-147 and miR-127-3p was determined by RT-qPCR and each miRNA expression level was normalized using global normalization method. The error bars showed the SD. The significance of differences for miRNA expression was calculated using two-tailed T-test and *, p < 0.05. D0: onset of estrous; D3: day 3 of estrous; D7: day 7 of estrous