Fig. 1
From: Progesterone acts via the progesterone receptor to induce adamts proteases in ovarian cancer cells
Distribution of AR, ER and PR in NIH-OVCAR-3 and ES-2 cells. AR, ER and PR (red), nuclei (blue) and actin (green). Merge dots in magenta indicate the colocalization of the receptors with the nucleus. In NIH-OVCAR-3 cells a Androgen receptor and b Estrogen receptor are mostly in the nucleus, but also appears in cytoplasm. c Progesterone receptor is mostly found in the cell cytoplasm. In ES-2 cells d Androgen receptor and e Estrogen receptor are also mainly in the cell nucleus. f Progesterone receptor is located in cytoplasm and nucleus. g Immunoblot of NIH-OVCAR-3 and ES-2 cell fractions for receptors. In NIH-OVCAR-3 cells ER and AR are present in the nucleus, PR is present in both compartments, but mostly in the cytosolic fraction. ES-2 cells present most of the AR and ER in the nuclear fraction and PR is preferentially in the cytosolic compartment. Scale bar: 20 μm. (C = cytosolic fraction, N = nuclear fraction)