Fig. 1

Morphology, AP activity, cell surface markers expression, cell cycle and proliferation of OvSCs and Oct4-OvSCs. a The morphology and AP activity of OvSCs (a a-a c), Oct4-OvSCs (a d-a g) and AFs (a h and a i). Purple brown color indicates the positive reaction of AP activity in OvSCs and Oct4-OvSCs. AFs showed no AP activity. Scale bar = 50 μm. b Expression of cell surface markers, CD29⁺, CD44⁺, CD90⁺ and CD45^-. Each sample was counted with 1 × 10⁴ cells by flow cytometer. c Cell cycle status, each sample was counted with 1 × 10⁴ cells by flow cytometer (*p < 0.05). d Cell proliferation at various time intervals (*p < 0.05). e Expression of GFP as a lentiviral infection control analyzed by flow cytometer in Oct4-OvSCs. OvSCs showed no GFP expression (Independent triplicates, Mean ± S.E.)