Fig. 5

HE4 promotes deregulation of mitogen activated protein kinase (MAPK) signaling. a Protein was collected from SKOV3-NV, SKOV3-C1, and SKOV3-C7 cells and OVCAR8-NV and OVCAR8-C5 cells and western blot performed for phosphorylated ERK and total ERK. b Densitometry analysis of phospho-ERK/ERK ratio, from western blot in (a). c SKOV3-WT and OVCAR8-WT cells were treated with 20 nM recombinant HE4 for the indicated times, or left untreated. Protein was collected and western blot performed to determine levels of phosphorylated ERK and total ERK. d Densitometry analysis of phospho-ERK/ERK ratio, from western blot in (c). e SKOV3-NV and SKOV3-C1 cells were treated with vehicle (DMSO) or 25 μM cisplatin for the indicated times and western blot performed to detect levels of phospho-p38 and total p38. f Densitometry analysis of phospho-p38/p38 ratio, from western blot in (e)