Comparison of quartz vials with polypropylene vials for rapid cryopreservation of human ovarian tissue

Table 1 Histomorphological analysis of preservation for follicles, stromal cells, and vessels in fresh (A) ovarian tissue, rapid frozen/thawed tissue in PP vial (B), and in quartz vial (C)

Preservation	Score	A - fresh ovarian tissue	B - rapid frozen/thawed tissue in PP vial	C - rapid frozen/thawed tissue in quartz vial	P (Fisher’s exact test)
Follicles	1	9 (90 %)	4 (36.4 %)	12 (92.3 %)	0.004*
	2	1 (10 %)	7 (63.6 %)	1 (7.7 %)
	3	0	0	0
Stromal cells	1	10 (62.5 %)	13 (54.2 %)	9 (47.4 %)	0.80
	2	6 (37.5 %)	9 (37.5 %)	8 (42.1 %)
	3	0 (0 %)	2 (8.3 %)	2 (10.5 %)
	4	0 (0 %)	0 (0 %)	0 (0 %)
Vessels	1	5 (38.5 %)	2 (10 %)	1 (9.1 %)	0.16
	2	6 (46.1 %)	7 (35.0 %)	6 (54.5 %)
	3	2 (15.4 %)	5 (25.0 %)	3 (27.3 %)
	4	0 (0 %)	6 (30.0 %)	1 (9.1 %)

For follicle preservation, score 1 is considered as well preserved, score 2 as quite good preserved, and score 3 as degenerated follicles. For stromal cells and vessels, scores 1 + 2 are considered as well preserved, score 3 as not optimal preservation, and score 4 as degenerated stromal cells or degenerated vessels, resp
Data are presented as numbers and percentage (in brackets)
*) Method B differs significantly from C (two sided Fisher’s exact test, p = 0.008)

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