Fig. 1From: Differential regulation of Janus kinase 3 (JAK3) in bovine preovulatory follicles and identification of JAK3 interacting proteins in granulosa cells JAK3 mRNA expression and regulation in bovine follicles. Total RNA extracts of GC from small follicles (SF), dominant follicles (DF), ovulatory follicles (OF), and corpus luteum at day 5 (CL) were analyzed by RT-PCR for JAK3 with GAPDH used as reference. a Gel analysis of JAK3 in different groups of follicles and CL and corresponding histograms. JAK3 mRNA expression was strongest in DF and was significantly decreased in OF and CL (P < 0.0001). b Gel analysis of JAK3 mRNA regulation in hCG-induced follicular walls (FW) isolated from OF at 0, 6, 12, 18 and 24 h (hrs) after hCG injection and corresponding histograms. JAK3 mRNA was markedly decreased in FW 6 h post-hCG compared to its expression before hCG treatment (P < 0.01) and reached its weakest expression 24 h post-hCG as compared to 0 h (P < 0.0001). c Gel analysis of JAK3 mRNA regulation by endogenous LH. Similar to hCG, a decrease in JAK3 expression was observed 24 h after endogenous LH (LH) surge as compared to 0 h. d Representative JAK3 protein expression and regulation in bovine follicles. Total protein extracts of GC from SF, DF, OF, and CL were analyzed by western blot using anti-JAK3 antibodies. The strongest JAK3 protein expression was observed in the DF while weakest expression was observed in OF reflecting the regulation of the mRNA. Data are presented as least-square means ± SEM, and the number of independent samples per group is indicated in parenthesisBack to article page