Fig. 5
From: Quantifying growing versus non-growing ovarian follicles in the mouse
Development of follicle counting scheme that accounts for GC proliferation/follicle growth. a For preantral follicles: i. If the oocyte is fragmenting or degenerate, score the follicle Non-growing/Atretic. 1. Count all GC mitotic figures in the center section that contains the oocyte nucleus (light blue). If >= 1 dividing GC, the follicle is scored Growing/Intact. Go to step 2. 2. If zero dividing GC in center section, count mitotic and pyknotic GC in sections 0, +1, then -1. If a total of 2 dividing GC is reached, the follicle is Growing/Intact. Go to step 3. 3. The follicle is Non-growing/Atretic if fewer than 2 dividing GC are found, or, dying cells >= dividing cells. b For antral follicles: For antral follicles, find the center section that contains the oocyte nucleus. If the oocyte is fragmenting or degenerate, score the follicle Non-growing/Atretic. Score round follicles Intact, and misshapen follicles Atretic