Fig. 6

Comparison of methods for quantification of immature follicles in PM/PM ovaries and WT controls. Total numbers of primordial, primary, secondary, small preantral, and antral follicles are shown as determined by the ‘Historical’ method (grey bars) and the method ‘Proposed’ here (white bars). No significant difference was seen in primordial, primary, secondary and small preantral follicle types between counting methods. The Proposed method resulted in significantly fewer antral follicles designated Intact than the Historical method (P <0.05, Student’s t-test)