Fig. 2

Targeting Notch signalling pathway regulates angiogenesis in the pathological ovary. Interaction of Notch receptors with Notch ligands, such as Delta-like or Jagged, between two endothelial tip cells and stalk cells leads to a cascade of proteolytic cleavages. Notch intracellular domain (NICD) is released from the cell membrane by γ-secretase complex. Then, NICD translocates to the nucleus, where it interacts with the DNA-binding protein RBP-Jkappa, thus further activating the transcription of Notch target genes. Further, endothelial cell are activated and network formation is induced during angiogenesis in pathological ovary. Different strategies have been used to block Notch signalling by using anti-Dll4 monoclonal antibodies, γ-secretase inhibitors, anti-Notch antibodies, or Notch1-trap. Given that crosstalking of VEGF and Notch play an important role in angiogenesis in pathological ovary, VEGF could be blocked by anti-VEGF antibodies