Fig. 3
Overexpression of let-7e decreases the DSB repair capacity of EOC cell lines treated with cisplatin. a DSB repair dynamics of transfected A2780 and OV2008 cells after treatment with cisplatin measured by neutral comet assay. The TM values measured at 0 h was set as 100% and residual DNA damage at 4, 8, 24 h after cisplatin incubation was expressed as percent of that at 0 h. Representative images are shown on the left and the mean ± SD for each group in the right panel. b After treatment with cisplatin, γ-H2AX foci in A2780 transfected with let-7e agomirs and SKOV3 with let-7e inhibitors were examined 8 h after recovery. The percentage of positive cells (with five or more foci) were used for the comparison between groups. Representative images and the mean ± SD for each group are shown. c and d Western bolt shows the BRCA1 and Rad51 levels of cell lines transfected with let-7e agomirs or inhibitors. e and f qRT-PCR analyses show the mRNA levels of PARP1 and IGF1 in cell lines transected with let-7e agomirs or inhibitors. Student’s t-test, *P < 0.05, **P < 0.01, ***P < 0.001