MicroRNA-130b is involved in bovine granulosa and cumulus cells function, oocyte maturation and blastocyst formation

Table 2 The proportion of oocytes (mean ± SD) at GV-arrest, metaphase l or telophase l stages in GV oocytes injected with miR-130b precursor, inhibitor or scramble miRNA and uninjected groups

Treatment groups	No of GV oocytes	GV-arrest	Metaphase l	Telophase l	Metaphase ll
miR-130b precursor	189	8.2 ± 0.1	8.3 ± 3.3	10.1 ± 5.5^a	73.5 ± 10.1^a
miR-130b inhibitor	204	8.9 ± 3.1	6.7 ± 3.9	30.1 ± 7.8^b	52.8 ± 4.4^b
Scramble	192	7.4 ± 0.9	6.8 ± 4.1	15.3 ± 7.8^a	69.7 ± 9.7^a
Uninjected control	191	7.3 ± 0.8	6.7 ± 2.8	13.0 ± 5.9^a	72.3 ± 6.1^a

Different letters of superscripts in the same column indicate significant difference (P ≤ 0.05) between treatment groups. GV Germinal vesicle, SD standard deviation

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