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Table 2 The proportion of oocytes (mean ± SD) at GV-arrest, metaphase l or telophase l stages in GV oocytes injected with miR-130b precursor, inhibitor or scramble miRNA and uninjected groups

From: MicroRNA-130b is involved in bovine granulosa and cumulus cells function, oocyte maturation and blastocyst formation

Treatment groups

No of GV oocytes

GV-arrest

Metaphase l

Telophase l

Metaphase ll

miR-130b precursor

189

8.2 ± 0.1

8.3 ± 3.3

10.1 ± 5.5a

73.5 ± 10.1a

miR-130b inhibitor

204

8.9 ± 3.1

6.7 ± 3.9

30.1 ± 7.8b

52.8 ± 4.4b

Scramble

192

7.4 ± 0.9

6.8 ± 4.1

15.3 ± 7.8a

69.7 ± 9.7a

Uninjected control

191

7.3 ± 0.8

6.7 ± 2.8

13.0 ± 5.9a

72.3 ± 6.1a

  1. Different letters of superscripts in the same column indicate significant difference (P ≤ 0.05) between treatment groups. GV Germinal vesicle, SD standard deviation