Fig. 4

Pathological observation of EOCs with TIC III. H&E staining of (grade 3) EOCs demonstrated that gland lumens had disappeared, tumor cells had larger nucleus and more distinct nucleoli (a). Brown microvessels with CD31 staining were observed with IHC method (b). IHC staining showed VEGF-positive expression displayed brownish yellow granules in the cytoplasm and intercellular spaces (c). IHC staining displayed yellow or brown granules appeared in Ki67-positive cell nuclei (d). (×200)