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Fig. 5 | Journal of Ovarian Research

Fig. 5

From: Macrophage colony-stimulating factor (M-CSF) is an intermediate in the process of luteinizing hormone-induced decrease in natriuretic peptide receptor 2 (NPR2) and resumption of oocyte meiosis

Fig. 5

Effect of M-CSF on NPR2 mRNA expression and oocyte maturation. COCs isolated from eCG-stimulated mice were cultured in MEM-α in the presence of 30 nM NPPC (control), to which was added 200 ng/ml M-CSF and/or 1 μmol/L GW2580 for 2 h. a Effect of GW2580 on M-CSF-induced NPR2 mRNA levels in cumulus cells after 2 h of culture. *, P < 0.05 compared with control. b Effect of GW2580 on M-CSF-induced resumption of oocyte meiosis after 2 h of culture. *, P < 0.05 compared with control. Bars demonstrate the mean ± SEM of three independent experiments with at least 10 COCs evaluated at each group in each experiment. c Presence of GVBD (hollow arrows) or intact germinal vesicle (GV, solid arrows) in oocytes at 2 h of COC culture. Scale bar, 25 μm. d Onset of meiotic resumption in oocytes (green arrows). GW, GW2580

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Journal of Ovarian Research

ISSN: 1757-2215

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