Table 1 Primer sequences for real-time RT-PCR
From: DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathway
Gene | Primer |
|---|---|
TGF-β | F: 5’-TACTGCTTCAGCTCCACAGAGA-3′ |
R: 5’-CAGACAGAAGTTGGCATGGTAG-3′ | |
CTGF | F: 5’-CATTAAGAAGGGCAAAAAGTGA-3′ |
R: 5’-CACACCCCACAGAACTTAGCC-3′ | |
Fibronectin | F: 5’-TGACAACTGCCGTAGACCTGG-3’ |
R: 5’-TACTGGTTGTAGGTGTGGCCG-3’ | |
MMP2 | F: 5’-CTTTGCAGGAGACAAGTTCTGG-3’ |
R: 5’-TTAAGGTGGTGCAGGTATCTGG-3’ | |
MMP9 | F: 5’-AAGCCTTGGTGTGGCACGAC-3’ |
R: 5’-TGGAAATACGCAGGGTTTGC-3’ | |
β- actin | F: 5’-TCAGGTCATCACTATCGGCAAT-3’ |
R: 5’-AAAGAAAGGGTGTAAAACGCA-3’ |