Fig. 10

Co-expression of NUMB and OCT-4 on dividing ovarian stem cells a Nuclear OCT-4A positive VSEL (arrow) undergoing asymmetric cell division (ACD) to give rise to OSC (asterisk). b Co-expression of OCT-4A and NUMB on different sizes of stem cells shown different expression pattern for (NUMB an OCT-4A). Please note that smaller stem cells express nuclear OCT-4A (red) with minimal expression of NUMB. Whereas slightly bigger progenitor cell show OCT-4A expression in cytoplasm but not in nucleus with clear uniform distinct expression pattern for NUMB (green) in cytoplasm. c Co-expression of NUMB and OCT-4 on progenitor ovarian stem cells undergoing symmetric cell division (SCD). Please note progenitor cells are of equal sizes and expresses similar expression pattern for (NUMB and cytoplasmic OCT-4A). d Negative control by omission of primary antibody showed no staining. Cells were counterstained with DAPI. OCT-4A is a nuclear transcription factor suggesting pluripotent state whereas NUMB is expressed by, progenitor OSC and is implicated to suppress Notch signaling essential for maintaining undifferentiated stem cells. Scale bar 10 μm