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Fig. 5 | Journal of Ovarian Research

Fig. 5

From: Investigation of hypoxia networks in ovarian cancer via bioinformatics analysis

Fig. 5

Validation of the selected genes in gene integration networks in Caov3 cells. Cells were exposed to hypoxia (treated with CoCl2 or transfected with hif-1α overexpression vector) followed by transiently transfected with shRNAs vector targeting TGFA, EGFR, or empty vector. Then total RNA and proteins were extracted for qRT-PCR and western blotting analysis. a and b, ErbB2 related mRNA expression; c and d, MYC related mRNA expression; E and F, ErbB2, MYC, GAPDH related protein expression, and GAPDH was used as a loading control. Values with different letters (ac) differ significantly (p < 0.05)

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