Fig. 5
From: Investigation of hypoxia networks in ovarian cancer via bioinformatics analysis
Validation of the selected genes in gene integration networks in Caov3 cells. Cells were exposed to hypoxia (treated with CoCl2 or transfected with hif-1α overexpression vector) followed by transiently transfected with shRNAs vector targeting TGFA, EGFR, or empty vector. Then total RNA and proteins were extracted for qRT-PCR and western blotting analysis. a and b, ErbB2 related mRNA expression; c and d, MYC related mRNA expression; E and F, ErbB2, MYC, GAPDH related protein expression, and GAPDH was used as a loading control. Values with different letters (a–c) differ significantly (p < 0.05)