Fig. 4

Analysis of SYCP3 expression following treatment of differentiations with RA for 48 h. a The expression level of SYCP3 was found to be significantly higher following exposure to 10 μm RA when compared to the vehicle only control or undifferentiated stem cells. b Immunolocalization of SYCP3 had a largely cytoplasmic localization in control differentiations (left two panels). In the RA treated cultures the location of SYCP3 (middle two panels) more closely resembled that of fetal ovary controls (right two panels), with chromosome localization. The data were analyzed using one-way ANOVA with Tukey’s post-hoc test (Data represent at least n = 3 biological replicates)