Quercetin increases the antioxidant capacity of the ovary in menopausal rats and in ovarian granulosa cell culture in vitro

Table 1 Characteristics of Primers and Real-Time PCR Conditions

Gene	Forward primer (5′ → 3′)	Reverse primer (5′ → 3′)	Accession number
SOD-1	AGGGCGTCATTCACTTCGAG	CCTCTCTTCATCCGCTGGAC	NM_017050.1
CAT	TTTTCACCGACGAGATGGCA	CTGACTCTCCAGCGACTGTG	NM_012520.2
GSS	GAGTTTGAGCTTGGCGAGCAG	ATGGGGCATACGTCACCAC	NM_012962.1
β-actin	CACCCGCGAGTACAACCTTC	CCCATACCCACCATCACACC	NM_031144

Two-step real-time PCR conditions: Stage 1: Initial denaturation = 30 s at 95 °C; Stage 2: PCR amplification, 5 s at 95 °C and 34 s at 60 °C; extension = 30 s at 72 °C

ISSN: 1757-2215