Fig. 2

In vivo imaging of ID8-L11 cells and effect of serum from wounded mice on growth of parental ID8 cells. a Schematic of the treatment paradigm used in the preliminary study. b Representative bioluminescence images of a mouse not injected with ID8-L11 cells (left) and a mouse injected with 5 million ID8-L11 cells i.p. 13 days earlier (right). c Representative images of the abdominal viscera of mice in each of the four treatment groups at time of necropsy. No evidence of macroscopic seeding was found. d Serum from mice of the different treatment groups did not impact the growth of parental ID8 cells in vitro. Cells were grown in medium supplemented to 10% with serum pooled from within the four treatment groups in the presence or absence of 25 μM cisplatin. XTT dye reduction assay was performed 72 h after initiation of treatment. Bars represent the mean ± SEM of 8 replicates. Two-way ANOVA performed on natural log-transformed data indicated a statistically significant effect of cisplatin but no statistically significant effect of serum from the different treatment groups or interaction