Table 2 Primer details
From: Characterization of stem cell and cancer stem cell populations in ovary and ovarian tumors
Gene | Primer Sequence | Annealing Temperature (°C) | Amplicon size (base pair) |
|---|---|---|---|
GAPDHa | F: 5-TGATGACATCAAGAAGGTGGT-3 | 60 | 240 |
R: 5-TCCTTGGAGGCCATGTGGGCC-3 | |||
ALDHb | F: 5-GCACGCCAGACTTACCTGTC-3 | 60 | 91 |
R: 5-CCACTCACTGAATCATGCCA-3 | |||
C-kitb | F: 5-GGCATCACGGTGACTTCAAT-3 | 62 | 244 |
R: 5-GGTTTGGGGAATGCTTCATA-3 | |||
CD133b | F: 5-AGTGGCATCGTGCAAACCTG-3 | 62 | 184 |
R: 5-CTCCGAATCCATTCGACGATAGTA-3 | |||
CD24b | F: 5-TGCTCCTACCCACGCAGATT-3 | 60 | 89 |
R: 5-GGCCAACCCAGAGTTGGAA-3 | |||
CD44b | F: 5-CCAATGCCTTTGATGGACCA-3 | 60 | 334 |
R: 5-TGTGAGTGTCCATCTGATTC-3 | |||
Ki67c | F: 5-GAGGTGTGCAGAAAATCCAAA-3 | 60 | 78 |
R: 5-CTGTCCCTATGACTTCTGGTTGT-3 |