Fig. 5

DAXX binds to CEBP-β and promotes ascites cell proliferation in vivo. a–b Immunohistochemistry staining for p-ERK1/2 and p-AKT in mouse tumor tissue. Scale bar, 200 μm. c DAXX-overexpressing ascites cells were subjected to immunoblotting analysis with antibodies against the indicated proteins. d DAXX-overexpressing ascites cells were transfected with the indicated combinations of plasmids encoding HA-CEBP-β. Cells were lysed after 24 h, and protein extracts were immunoprecipitated with anti-HA antibodies, followed by SDS-PAGE and western blotting. e Immunohistochemical analysis of DAXX and CEBP-β levels in human ovarian tumor tissue samples (n = 10). Sections were counterstained with hematoxylin and eosin. Scale bar, 200 μm. f Cebpβ, Tff1 and p16 mRNA expressions in ES-Daxx, I and II ascites cells were determined by Q-PCR