Table 1 Primer sets used in quantitative RT-PCR
From: Aberrant DNA methylation suppresses expression of estrogen receptor 1 (ESR1) in ovarian endometrioma
Name | Forward |
|---|---|
ESR1 | |
 all variants | F: 5′-TGTGCAATGACTATGCTTCA-3′ |
 variant1 (transcribed from uExon-A) | F: 5′-CTCGGGCTGTGCTCTTTTT-3′ |
 variant2 (transcribed from uExon-B) | F: 5’-GCCGTGAAACTCAGCCTCTA-3′ |
 variant3 (transcribed from uExon-C) | F: 5′-TGGAACATTTCTGGAAAGACG-3′ |
ESR2 | |
 variant-a/variant-b | F: 5′ -CTCGCTTTCCTCAACAGGTG- 3′ |
PGR | |
 variant1/variant2 | F: 5′ -ACCAGCTCTTGGTGCCTGT- 3′ |
 variant2 | F: 5′ -TCCCTCTGCCCCTATATTCC- 3′ |
GAPDH | F: 5′-AGGTGAAGGTCGGAGTCA-3′ |