Skip to main content

Table 1 Primer sets used in quantitative RT-PCR

From: Aberrant DNA methylation suppresses expression of estrogen receptor 1 (ESR1) in ovarian endometrioma

Name Forward
ESR1
 all variants F: 5′-TGTGCAATGACTATGCTTCA-3′
 variant1 (transcribed from uExon-A) F: 5′-CTCGGGCTGTGCTCTTTTT-3′
 variant2 (transcribed from uExon-B) F: 5’-GCCGTGAAACTCAGCCTCTA-3′
 variant3 (transcribed from uExon-C) F: 5′-TGGAACATTTCTGGAAAGACG-3′
ESR2
 variant-a/variant-b F: 5′ -CTCGCTTTCCTCAACAGGTG- 3′
PGR
 variant1/variant2 F: 5′ -ACCAGCTCTTGGTGCCTGT- 3′
 variant2 F: 5′ -TCCCTCTGCCCCTATATTCC- 3′
GAPDH F: 5′-AGGTGAAGGTCGGAGTCA-3′