Fig. 3
Analysis of cytopathic effects of replication competent Ad vectors. a Cytotoxic effects of wild type Ad5 were compared in A549, GL261 and F3 using CellTox™ assay. Each data point represents the cumulative mean of triplicate measurements ± SD (p ≤ 0.05 *). b Monolayers of A549, GL261 and F3 were infected with indicated Ad vectors and cell viability measured after 5 days by adding CellTiter 96 AQueous One Solution Reagent. The percentages of live cells in the monolayers exposed to replication-deficient E1-deleted Ad5∆E1 are shown as compared to wild type Ad5-infected cells. c Replication was assed 48 h after infection at MOI 100vp/cell and 1000vp/cell using the Adeno-X-Rapid Titer kit. Final titers were determined at pfu/mL (p ≤ 0.05 *)