Fig. 2From: HAS2-AS1 is a novel LH/hCG target gene regulating HAS2 expression and enhancing cumulus cells migrationHAS2-AS1 effect on HAS2 expression. KGN cells were transfected with HAS2-AS1 siRNA or scrambled control. After 48 h cells were harvested and subjected to total RNA purification, cDNA synthesis and qRT-PCR with HAS2 and HAS2-AS1 primers. Results are means ± SEM of three independent experiments normalized to b-actin expressionBack to article page