Fig. 3

KGN cells, transfected with HAS2-AS1 siRNA or scrambled control, were grown at 95–100% confluency and starved overnight (12-14 h) in serum-free medium. During the scratch assay, FBS in the medium was set to 0.2% to inhibit apoptosis or necrosis, and at the same time, inhibit proliferation. A midline vertical scratch was performed using a 10 μl tip with a blunt surface. Images were taken 0 h, 18 h, and 27 h after incubation. Each bar represents 500 mm. The dotted lines define the areas lacking cells. Images reflect a representative experiment out of a total of at least three independent experiments