Fig. 2

Immmunohistochemical staining on normal and cancerous chicken ovarian tissue for angiogenic markers a Normal and cancerous chicken ovaries from control, whole flax, defatted flax meal and flax oil groups were stained with anti-CD31, anti-⍺ smooth muscle actin, anti-vascular endothelial growth factor, anti-vascular endothelial growth factor receptor type 2 antibodies and NG2 co-stained with CD31. b Quantified expression of the angiogenic markers, number of positive cells over total number of cells per field of view. Two-way ANOVA, error bars: SD, p < 0.05