Fig. 4
2MeOE2 treatment of human ovarian cancer cells a BG1, HeyC2 and TOV112D cells were treated with 10 μM 2MeOE2, 10 μM SB203580 and 10 μM 2MeOE2 and SB203580 together. Cells were photographed after 24 h from treatment. 10 μM 2MeOE2 plates had significant reduction in cell numbers from the control plate. b Western blot analysis performed on protein lysates from control and 10 μM 2MeOE2 against p38 and phospho-p38 on BG1, HeyC2 and TOV112D cells (n = at least 3 for each dataset). One-way ANOVA, error bars: SEM, p < 0.05. c Western blot analysis performed on protein lysates from control, 10 μM 2MeOE2, 10 μM SB203580 and 10 μM 2MeOE2 + 10 μM 2MeOE2 against caspase 3 and cleaved caspase 3 on BG1, HeyC2 and TOV112D cells (n = 3). Amount of caspase-3 cleaved into the active cleaved caspase-3 was quantified to estimate the amount of apoptosis induced by each treatment. Two-way ANOVA, error bars: SEM, p < 0.05