Fig. 4

Splicing alteration was identified by a minigene assay. (a) Amplification bands of colony PCR tests in pcDNA3.1-wt/mut. (b) Sequencing in the recombinant vector. The top of Fig. 4b indicates the results of WT minigene sequencing, and the bottom shows the sequencing of the MT (c.3470G > A) minigene. Both of them are partial sequencing results. The red frame indicates the base changed by the mutation. (c) Schematic diagram of minigene construction. (d) Reverse-transcription polymerase chain reaction (RT-PCR) products were separated by electrophoresis of the pcDNA3.1 vector in HeLa and c293T cells. (e) Schematic diagram of Sanger sequencing of RT-PCR products. (f) The sequencing results for the bands