Fig. 3

Expression of Tumor markers. a-d CD44+/CD24- expression in CAOV3, OVCAR3, IGROV3. e-h Cell culture supernatant from co-culture was collected, and undiluted samples were analyzed for detection of CA-125, LDH and beta-hCG. The concentration of secretion is shown in the x-axes. The limits of detection (IU/ml) were CA-125: 0.95, beta-HCG: 0.99, LDH = 0.99. i Real time PCR showed a decrease in Ca-125 levels in CAOV3, SKOV3 and OVCAR3, in mRNA levels. The results were displayed as percentage of controls. Error bars represent the means ± SD, n = 5; * p = 0.051, **p = 0.005, ***p < 0.001,****p < 0.0001