Fig. 3

AB209371 up-regulate Survivin by down-regulating miR-203. UWB1.289 cells were transfected with AB209371 and Survivin vectors as well as miR-203 mimic. At 24 h post-transfection, expression levels of AB209371, Survivin mRNA and miR-203 were measured and over-expression was confirmed by qPCR (a). Effects of AB209371 and miR-203 over-expression on Survivin mRNA in OC cells were analyzed by qPCR (b). Effects of AB209371 and miR-203 over-expression on Survivin protein in OC cells were analyzed by Western blot, and the representative images were showed in (c). Quantification data of western blot were shown in (d). The interaction between miR-203 and AB209371 was analyzed by qPCR (e). Mean values were presented, *, p < 0.05