Fig. 3

The influence of PLC inhibitor and activator on apoptosis of porcine granulosa cells in vitro. The cells were challenged with 0.5 μM U73122 or m-3M3FBS for 4 h (genes) or for the times given (percentage of apoptotic cells), which were processed for annexin V/PI staining and measured by flow cytometry assay. (A) The effect of U73122 on the abundance of the mRNAs that encode the transcription factors involved in the regulation of apoptosis of porcine granulosa cells. (B) The percentage of early apoptosis and late apoptosis treated with 0.5 μM U73122 at different times. (C) The effect of m3M3FBS on the abundance of the mRNAs that encode the transcription factors involved in the regulation of apoptosis of porcine granulosa cells. (D) The percentage of early apoptosis and late apoptosis treated with 0.5 μM m-3M3FBS at different times. Data are mean ± S.E.M. of three independent replicates. Independent sample t-test was used for (A) and (C), and one-way ANOVA was used for (B) and (D). For each treatment, means without asterisk are not significantly different, means with * indicate a difference at p <0.05 compared with control, means with ** indicate a difference at p <0.01 compared with control (A and C); or means without common letters are significantly different(p<0.05) (B and D)