Table 1 Overview of cancer cell culture techniques, adapted from [11,12,13]
From: Organoids of epithelial ovarian cancer as an emerging preclinical in vitro tool: a review
Technique | Advantages | Disadvantages |
|---|---|---|
Traditional cell culture (monolayer and spheroids) | - Easily maintained and expanded | - Cancer cells lose grow potential in vitro |
- Genetic manipulation | - Often only one clone expands | |
- High-throughput drug screening | - Lower biological stability | |
- Most favorable cost-benefit | - Inability to represent the clinical cancer spectrum | |
Xenografts | - In vivo culture | - Difficult genetic manipulation |
- High heterogeneity possible | - Graft failure | |
- Complete microenvironment | - Time and resource consuming | |
- Lack of high-throughput drug screening | ||
Organoids | - Long term expansion | - Unknown capability to capturing whole spectrum of in vivo heterogeneity |
- Mimicking in vivo conditions | - Time consuming initiating a model | |
- High-throughput drug screening | - No microenvironment | |
- Tissue subtype modeling | Â |